胺鹽Spermine

胺鹽Spermine
Product Number S3256
Store at 2-8 °C
Product Description
Molecular Formula: C 10 H 26 N 4
Molecular Weight: 202.34
CAS Number: 71-44-3
Melting Point:  55 - 60 °C 1
28 - 30 °C 2
Synonym: N,N'-bis(3-aminopropyl)-1,4-butanediamineSpermine is a naturally occuring polyamine that occursin all eukaryotes, but is rare in prokaryotes. It isessential for cell growth in both normal and neoplastictissue. 1 Spermine is formed through the addition of aaminopropyl group to spermidine by sperminesynthase. Spermine is strongly basic in character, and in aqueous solution at physiological pH, all of its amino groups will be positively charged. 3 A review of the role of spermine and other polyamines in affecting RNA structure and protein function has been published. 4 Spermine is commonly used in molecular biology and biochemistry research. The polycationic character of spermine in solution allows for its use in the precipitation of DNA of >100 base pairs in length from low salt aqueous buffers, and for the isolation of DNA from pulse field gels. 5,6,7 Spermine has also been utilized in chromosome isolation and in the aggregation of chromatin. 8,9 It may be used as a building block for the preparation of gene transfer agents. 10,11 The complexation of spermine with DNA
to form particles with diameter <100 nm has been studied. 12 Spermine has been used in the crystallization of DNA. 13,14
It has been reported that spermine interacts with the glycine binding site associated with the N-methyl- D-aspartate receptor complex. 15 Spermine has been
shown to inhibit the conversion of L-arginine to L-citrulline by neuronal nitric oxide synthase in cytosolic preparations of rat cerebellum and cultured cerebellar granule neurons. 16 In acute hippocampal slices, 1 mM spermine exerts a neuroprotective effect
by blocking the NMDA receptor and voltage-activated Ca 2+ channels. 17 The effect of spermine on MK-801 binding to recombinant N-methyl-D-aspartate receptors has been investigated. 18 Proteins and protein complexes have been crystallized using spermine. 19,20 Other applications of spermine include its use as a matrix in MALDI-MS for analysis of glycoconjugates and oligonucleotides. 21,22 Precautions and Disclaimer For Laboratory Use Only. Not for drug, household or other uses.
Preparation Instructions
This product is soluble in water (50 mg/ml), yielding a clear, colorless to light yellow solution. Storage/Stability Store at 2-8 °C. Solutions of spermine free base are readily oxidized. Solutions are most stable if prepared in degassed water and stored in frozen aliquots, under argon or nitrogen gas. References
1. The Index, 13th ed., Entry# 8817.
2. -Aldrich QC history for spermine.
3. Methods in Molecular Biology, Volume 79:Polyamine Protocols, Morgan, D. M. L., ed.Humana Press (Totowa, NJ: 1998), pp. 3-30.
4. Igarashi, K., and Kashiwagi, K., Polyamines:mysterious modulators of cellular functions.Biochem. Biophys. Res. Commun., 271(3), 559-564 (2000).
5. Basic Methods in Molecular Biology, 2nd ed.,Davis, L. G., et al., Appleton & Lang (Norwalk, CT:1994), pp. 179-180.
6. Wallace, D. M., Precipitation of nucleic acids.Methods Enzymol., 152, 41-48 (1987).
7. Molecular Cloning: A Laboratory Manual, 3rd ed.,Sambrook, J. and Russell, D. W., CSHL Press(Cold Spring Harbor, NY: 2001), pp. .5.86-5.88.
8. Cram, L. S., et al., Polyamine buffer for bivariatehuman flow cytogenetic analysis and sorting.Methods Cell Biol., 33, 377-382 (1990).
9. Marquet, R., et al., The condensation of chromatinand histone H1-depleted chromatin by spermine.J. Biomol. Struct. Dyn., 4(2), 205-218 (1986).
10. Ronsin, G., et al., Novel spermine-based cationic gemini surfactants for gene delivery. Chem.Commun. (Camb.), 21, 2234-2235 (2001).
11. Azzam, T., et al., Polysaccharide-oligoamine based conjugates for gene delivery. J. Med. Chem., 45(9), 1817-1824 (2002). 12. Trubetskoy, V. S., et al., The role of a microscopic colloidally stabilized phase in solubilizing oligoamine-condensed DNA complexes. Biophys. J., 84(2), 1124-1130 (2003).

13. Shui, X., et al., The B-DNA dodecamer at high resolution reveals a spine of water on sodium. Biochemistry, 37(23), 8341-8355 (1998).
14. Saminathan, M., et al., Polyamine structural effects on the induction and stabilization of liquid crystalline DNA: potential applications to DNA packaging, gene therapy and polyamine therapeutics. Nucleic Acids Res., 30(17), 3722- 3731 (2002).
15. Sacaan, A. I., and Johnson, K. M., Spermine enhances binding to the glycine site associated with the N-methyl-D-aspartate receptor complex. Mol. Pharmacol., 36(6), 836-839 (1989).
16. Hu, J., et al., Polyamines Inhibit Nitric Oxide Synthase in Rat Cerebellum. Neurosci. Lett., 175(1-2), 41-45 (1994).
17. Ferchmin, P. A., et al., Spermine is neuroprotective against anoxia and N-methyl-D- aspartate in hippocampal slices. Brain Res., 859(2), 273-279 (2000).
18. Sharma, T. A., and Reynolds, I. J., Characterization of the effects of polyamines on
[ 125 I]MK-801 binding to recombinant N-methyl-D- aspartate receptors. J. Pharmacol. Exp. Ther., 289(2), 1041-1047 (1999).
19. Xie, X., et al., Coiled-coil packing in spermine- induced tropomyosin crystals. A comparative study of three forms. J. Mol. Biol., 236(4), 1212- 1226 (1994).
20. Qian, C., et al., A rational approach towards successful crystallization and crystal treatment of human cytomegalovirus protease and its inhibitor complex. Acta Crystallogr. D Biol. Crystallogr., 56 (Pt 2), 175-180 (2000).
21. Mechref, Y., and Novotny, M. V., Matrix-assisted laser desorption/ionization mass spectrometry of acidic glycoconjugates facilitated by the use of spermine as a co-matrix. J. Am. Soc. Mass Spectrom., 9(12), 1293-1302 (1998).
22. Asara, J. M., and Allison, J., Enhanced detection of oligonucleotides in UV MALDI MS using the tetraamine spermine as a matrix additive. Anal. Chem., 71(14), 2866-2870 (1999). GCY / RXR 1/06

產(chǎn)品訂購(gòu)說(shuō)明：
1、絕大部分產(chǎn)品備有現(xiàn)貨，一般情況下都能訂貨當(dāng)日發(fā)貨。 
2、部分非常用產(chǎn)品，需提前1－2日預(yù)訂，海外期貨則需要提前3-6周預(yù)訂。 
3、部分產(chǎn)品價(jià)格會(huì)因貨期、批次等因素發(fā)生變化，若有變動(dòng)以訂貨當(dāng)日新價(jià)格為準(zhǔn)。 
4、每日訂單截止時(shí)間為16點(diǎn)整，部分城市可到17點(diǎn)整，因超過(guò)截止時(shí)間造成當(dāng)日不能發(fā)貨的將于次日安排發(fā)貨。
5、請(qǐng)辦理完貨款后，將收據(jù)、底單等連同收貨人的地址、姓名、等以傳真、、短信、等形式通知我們。
產(chǎn)品運(yùn)輸說(shuō)明：
極低溫產(chǎn)品：極低溫產(chǎn)品運(yùn)輸過(guò)程中加裝干冰運(yùn)輸。用干冰把產(chǎn)品包裹起來(lái)，再用泡沫盒密封，膠帶層層粘住泡沫盒，放入索萊寶的箱子，然后交付給合作快遞，安全快速高效放心的送客戶(hù)的手中，保證產(chǎn)品的性質(zhì)穩(wěn)定如一，為您的實(shí)驗(yàn)保駕護(hù)航。
低溫產(chǎn)品：低溫產(chǎn)品運(yùn)輸過(guò)程中加裝索萊寶冰袋運(yùn)輸。事先用冰袋把產(chǎn)品包裹起來(lái)，再使用泡沫盒密封，用膠帶嚴(yán)實(shí)密封泡沫盒，再放入索萊寶的箱子（保溫效果少可以持續(xù)一周），然后交付給合作快遞，安全快速高效放心的送客戶(hù)的手中，保證產(chǎn)品的性質(zhì)穩(wěn)定如一，為您的實(shí)驗(yàn)保駕護(hù)航。
常溫產(chǎn)品：常溫產(chǎn)品運(yùn)輸過(guò)程中無(wú)需加冰或者特殊包裝。產(chǎn)品由公司庫(kù)房人員快速配貨，準(zhǔn)確快速高效的快遞保證產(chǎn)品快速送達(dá)您的手中。
公司簡(jiǎn)介：
北京索萊寶科技有限公司始創(chuàng)于2000年，是一家集產(chǎn)品研發(fā)、生產(chǎn)、銷(xiāo)售、服務(wù)于一體的大型高科技生物企業(yè)。
公司總部坐落于北京中關(guān)村科技園區(qū)，擁有1200平米研發(fā)生產(chǎn)中心，2000平米倉(cāng)儲(chǔ)基地，并配有的物流系統(tǒng)。同時(shí)在全國(guó)30多個(gè)省市設(shè)有分公司及直銷(xiāo)、分銷(xiāo)機(jī)構(gòu)。服務(wù)網(wǎng)絡(luò)覆蓋全國(guó)，是國(guó)內(nèi)大的生命科學(xué)研發(fā)試劑供應(yīng)商之一。并通過(guò)阿里巴巴將產(chǎn)品輸入美、日、歐等發(fā)達(dá)國(guó)家，得到了市場(chǎng)的高度認(rèn)可。
目前公司提供的產(chǎn)品近萬(wàn)種，超過(guò)5000種常備現(xiàn)貨，涵蓋分子生物學(xué)、細(xì)胞生物學(xué)、免疫學(xué)、生物醫(yī)學(xué)等相關(guān)領(lǐng)域，并不斷推陳出新擴(kuò)充種類(lèi)，為用戶(hù)提供、專(zhuān)業(yè)的產(chǎn)品。公司擁有數(shù)十名由博、碩士組建的專(zhuān)業(yè)研發(fā)技術(shù)團(tuán)隊(duì)，并聘請(qǐng)了眾多國(guó)內(nèi)專(zhuān)家擔(dān)任技術(shù)顧問(wèn)。嚴(yán)格按照G M P和I SO 9000標(biāo)準(zhǔn)生產(chǎn)和管理，致力于為用戶(hù)提供試劑，完善的解決方案，致力于打造*的民族品牌。“為科研服務(wù)，為生命盡責(zé)”。是每一個(gè)索萊寶人發(fā)自?xún)?nèi)心的服務(wù)理念。同時(shí)，我們建立了一套的企業(yè)-高校合作模式，共同利用彼此平臺(tái)和優(yōu)勢(shì)，互通有無(wú)，并為高校學(xué)生提供各項(xiàng)實(shí)驗(yàn)技能培訓(xùn)，實(shí)現(xiàn)雙贏。此時(shí)，我們也期待能與更多科研單位深入合作，共同為中國(guó)生命科學(xué)研究的發(fā)展而努力，互惠共贏。
Solarbio品牌的誕生，是中國(guó)民族企業(yè)在生命科學(xué)領(lǐng)域的一次大膽挑戰(zhàn)，面對(duì)幾乎被外國(guó)企業(yè)壟斷的市場(chǎng)，面對(duì)嚴(yán)酷的競(jìng)爭(zhēng)環(huán)境，索萊寶人依然堅(jiān)定不移的走自主品牌戰(zhàn)略，立足國(guó)內(nèi)，放眼，為中國(guó)生命科學(xué)產(chǎn)業(yè)的發(fā)展默默的付出自己的努力。同時(shí)，我們也相信，不久的未來(lái)中國(guó)民族生物企業(yè)也一定能夠屹立于世界！